Composite
Part:BBa_K2066119:Experience
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-14)
This synthetic enhancer part is sequence confirmed.
Applications of BBa_K2066119
This synthetic enhancer part includes a 2x TetO binding cassette in the spacer region between the enhancer and sigma 54 promoter, and depending on the steady state of TetR, the rigidity of DNA changes and thus affects the thermodynamic capability of looping and activation of transcription.
Synthetic enhancer sequences were derived from Amit et. al. 2011 and UNS sequences from Torella et. al. 2012.
For more information on the mechanism of the synthetic enhancer circuit, refer to our page: http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer.